Timelapse Microscopy

the beauty of 3D timelapse microscopy

Early Zebrafish embryonic development (h2afva labeling)

Early Zebrafish embryonic development, slower rotation, different color (h2afva labeling)

Spatiotemporally adaptive imaging of Drosophila embryonic development.

21-h time-lapse experiment using spatiotemporally adaptive imaging. Dorsoventral maximum-intensity projections of a D. melanogaster embryo expressing RFP in all cell nuclei (w;His2Av-mRFP1;+). Two views correspond to two sliced renderings of the dorsal and ventral sides.

Spatiotemporally adaptive imaging of dynamic gene expression patterns.

Dorsoventral maximum-intensity projections of a D. melanogaster embryo expressing RFP in all cell nuclei and GFP in the nervous system (deadpanEE–Gal4, UAS–myr::GFP, His2Av–RFP), representing a 20-h time-lapse experiment using spatiotemporally adaptive imaging.

Spatiotemporally adaptive imaging of Drosophila embryonic development.

21-h time-lapse experiment using spatiotemporally adaptive imaging. Dorsoventral maximum-intensity projections of a D. melanogaster embryo expressing RFP in all cell nuclei (w;His2Av-mRFP1;+)

Spatiotemporally adaptive whole-brain functional imaging in larval zebrafish Tg(elavl3:GCaMP6f)

Adaptive light-sheet microscopy for long-term, high-resolution imaging in living organisms
Loïc A Royer, William C Lemon, Raghav K Chhetri, Yinan Wan, Michael Coleman, Eugene Myers & Philipp J Keller

http://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt.3708.html

Adaptive light-sheet microscope adapting to a growing mouse embryo

In Toto Imaging and Reconstruction of Post-Implantation Mouse Development at the Single-Cell Level
Katie McDole, Léo Guignard, Fernando Amat, Andrew Berger, Grégoire Malandain, Loïc A. Royer, Srinivas C. Turaga, Kristin Branson, Philipp J. Keller
https://www.cell.com/cell/pdf/S0092-8674(18)31243-1.pdf

First cell divisions of a C. elegans embryo (HisGFP & BTubGFP)

ClearVolume: open-source live 3D visualization for light-sheet microscopy
Loic A Royer, Martin Weigert, Ulrik Günther, Nicola Maghelli, Florian Jug, Ivo F Sbalzarini & Eugene W Myers